Influence of NF-κB on the development of oxidative-nitrosative stress in the liver of rats under conditions of chronic alcohol intoxication

Alcohol-related liver disease is the most common cause of worldwide. The purpose this work establishment influence transcription factor κB on development oxidative-nitrosative stress in rats under conditions chronic alcohol intoxication. experiments were performed 24 male Wistar weighing 180-220 g. animals divided into 4 groups 6 animals: control; animals, which administered NF-κB inhibitor, namely ammonium pyrrolidinedithio­carbamate (PDTC) at a dose 76 mg/kg 3 times week; we simulated alcoholic hepatitis and group combination inhibitor. We determined rat homogenate following biochemical parameters: activi­ty NO synthase isoforms, superoxide dismutase catalase activity, concentration malonic dialdehyde, peroxynitrite, nitrites nitrosothiols, sulfide anion radical production. Chronic intoxication led to increased production reactive oxygen nitrogen species background decreased antioxidant thus intensifying lipid peroxidation liver. Blockade during despite an increase activity decrease did not ameliorate oxidative damage activation nuclear by PDTC reduced risk hepatocytes, but reduce developing nitrosative hepatocytes. Keywords: intoxication, NF-κB, stress, PDTC,